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Product Description
Demo Units Available
The Mastercycler® ep realplex combines the highest performance and the most advance features of a 96 well real time PCR system. Select between two available detection systems. All systems are equipped with a 96 LED array to excite the fluorescent dyes in each individual PCR sample. The intuitive software interface ensures a simple, fast PCR and assay setup.
Features
- 96 individual LED array for even excitation across the whole thermoblock with lifetime warranty
- High speed real-time PCR , silver block up to 8°C/s
- Fast optical detection capabilities eliminate the need for lengthy extension/elongation
- No restrictions—use reagents, kits and consumables of your choice.
- SteadySlope® gradient technology and Triple Circuit Technology allows optimization in a single experiment
- Realplex software is intuitive and user friendly including six evaluation models: plate layout interface, raw data monitoring, quantification and relative quantification, gene identification, endpoint detection, and ± assay
- Two channel system with emission at 520/550nm
- Four-channel system with emission at 520/550/580/605nm provides full flexibility for multiplexing
| Mastercycler ep realplex2 | Mastercycler ep realplex2 S | Mastercycler ep realplex4 | Mastercycler ep realplex4 S | |
|---|---|---|---|---|
| Sample capacity |
96x 0.2 mL PCR tubes or 1 PCR plate 8 x 12 (unskirted,semi-skirted,skirted-according to SBS standard) |
96x 0.2 mL PCR tubes or 1 PCR plate 8 x 12 (unskirted,semi-skirted,skirted-according to SBS standard) |
96x 0.2 mL PCR tubes or 1 PCR plate 8 x 12 (unskirted,semi-skirted,skirted-according to SBS standard) |
96x 0.2 mL PCR tubes or 1 PCR plate 8 x 12 (unskirted,semi-skirted,skirted-according to SBS standard) |
| Thermoblock | Aluminum | Silver | Aluminum | Silver |
| Temperature control range |
4-99°C | 4-99°C | 4-99°C | 4-99°C |
| Gradient range | 1-20°C | 1-24°C | 1-20°C | 1-24°C |
| Gradient temperature range |
30-99°C | 30-99°C | 30-99°C | 30-99°C |
| Lid temperature range |
105°C | 105°C | 105°C | 105°C |
| Block homogeneity | 20-72°C < ±0.3°C, 90°C < ±0.4°C | 20-72°C < ±0.3°C, 90°C < ±0.4°C | 20-72°C < ±0.3°C, 90°C < ±0.4°C | 20-72°C < ±0.3°C, 90°C < ±0.4°C |
| Control accuracy | ±0.2°C | ±0.2°C | ±0.2°C | ±0.2°C |
| Heating rate | 4°C/s | 6°C/s | 4°C/s | 6°C/s |
| Cooling rate | 3°C/s | 4.5°C/s | 3°C/s | 4.5°C/s |
| Excitation source | 96 LEDs (470 nm) | 96 LEDs (470 nm) | 96 LEDs (470 nm) | 96 LEDs (470 nm) |
| Emission filters | 520 nm/550 nm | 520 nm/550 nm | 520/550/580/605 nm | 520/550/580/605 nm |
| Detector | 1 photomultiplier tube | 1 photomultiplier tube | 2 photomultiplier tubes | 2 photomultiplier tubes |
| Dynamic range | 8 orders of magnitude from starting copy number | 8 orders of magnitude from starting copy number | 8 orders of magnitude from starting copy number | 8 orders of magnitude from starting copy number |
| Sensitivity | <50 fmol fluorescein | <50 fmol fluorescein | <50 fmol fluorescein | <50 fmol fluorescein |
| Dimensions (W x D x H) |
10.24 x16.14 x 15.59 in | 10.24 x16.14 x 15.59 in | 10.24 x16.14 x 15.59 in | 10.24 x16.14 x 15.59 in |
| Weight | 52.91 lb | 52.91 lb | 52.91 lb | 52.91 lb |
| Power supply | 120 V, 50/60Hz | 120 V, 50/60Hz | 120 V, 50/60Hz | 120 V, 50/60Hz |
| Power consumption | 800 W | 800 W | 800 W | 800 W |
