The Eppendorf BioPhotometer® plus, a compact UV/Vis photometer, has 9 fixed wavelengths optimized for routine applications in molecular biology, biochemistry and cell biology labs. The photometer provides instant access to 32 routine methods, of which 9 methods are freely programmable. Apart from standard cuvettes such as the Eppendorf UVette, the Biospectrometer can also accommodate micoliter volumes.
- Requires no PC to operate
- Measurement of single wavelengths without any calculation
- Storage of the last 100 results and all corresponding data
- Simple user guidance for error-free operation
- Compact design and robust housing
- No pre-warming required
- Short measuring time
- High intensity Xenon flash lamp virtually lasts a lifetime
- 2-year warranty
Absorption single-beam photometer with reference
|Light source||Xenon flash lamp|
|Light beam height||8.5 mm|
|Spectral bandwidth||5 nm at 230-280 nm|
|±1 nm at 230-280 nm, ±2nm at 340-650 nm|
0 to 3 A (2 A at 340 nm); Dye methods: 2 A at 550
|±0.002 A at 0 A; ±0.005 A at 1 A|
|±1% at 1 A|
-absorbance, concentration via factor,
concentration via calibration with 1 to 10
standards, one-point calibration (1 standard),
linear regression (2 to 10 standards), non-linear
regression, ratio 260/280, ratio 260/230, molar
concentration, total yield,
|Calibration memory||For all calibration procedures|
For 100 results with absorbance and ratio values,
sample number, sample dilution, date and time
|Interface||RS-232 C, serial, PC connection optional|
Approx. 20 W in operation, approx. 10 W in standby
|Power supply||100/240 V, 50/60 Hz|
|Dimensions (W x D
|8.0 x 12.5 x 4.0 in|
|Weight||Approx. 6.6 lb|
|Accessories||Thermal printer DPU 414, Secondary -VIS filter set|
UVette, Hellma TrayCell and common regular
cuvettes of suitable glass or plastic materials
Application 228 BioPhotometer plus UVette Reproducible photome
Reproducible photometric determination of DNA concentrations using the Eppendorf UVette® in the Eppendorf BioPhotometer plus™
|English (US)||1.22 MB||download|
Userguide 010 BioPhotometer plus UV VIS Filter Evaluating the funct
Evaluating the functionality of the Eppendorf BioPhotometer plus using the Secondary UV-VIS Filter Set
|English (US)||191.27 kB||download|
Flyer BioPhotometer plus UVette The sixty seconds sm
The sixty seconds smile effect - Eppendorf BioPhotometer ™ plus and UVette ®
|English (US)||433.12 kB||download|
Operating manual BioPhotometer plus
||English (US)||3.61 MB||download|
I cannot find the method key “Bradford” for protein determination in the BioPhotometer plus! Is this method still available in the BioPhotometer plus?All methods of the BioPhotometer are still maintained. Furthermore in the BioPhotometer plus more methods are available compared to the BioPhotometer. Therefore a reorganization of the keypad was necessary: All related methods are summarized in one group of methods. For instance, in the method group “Protein” you will find all methods for protein determination like the colorimetric assays Bradford, Lowry, BCA as well as protein direct measurements.
. How many methods are available in the BioPhotometer plus in contrast to the BioPhotometer?With the BioPhotometer plus 32 methods (23 programmed and 9 freely programmable methods) can be used. In the precursor BioPhotometer only 9 methods were available.
What are the additional wavelengths that I can use with the new BioPhotometer plus in contrast to the old BioPhotometer?With the new BioPhotometer plus additional wavelengths at 340 nm, 405 nm, 490 nm, 550 nm and 650 nm can be applied. Two of these wavelengths have been changed: 340 nm instead of 320 nm, 550 nm instead of 562 nm. These changes do not effect the performance of any method.
I am working with dsDNA method. I have seen in the result display of the BioPhotometer plus that the turbidity correction is carried out at 340 nm instead of 320 nm. Why?Yes, this is correct. Due to this change more applications are possible with the BioPhotometer plus, e.g., endpoint measurement of NADH based enzyme assays. The turbidity correction at DNA, RNA and protein measurements can also be carried out at 340 nm with the same sensitivity.
What is the meaning of the “Dye 550” and “Dye 650” method at the BioPhotometer plus? .These methods are needed for the detection of fluorescent dye labeled biomolecules, like DNA, RNA or protein. With this application it is possible to check the quality of probes that should be used for microarray experiments. The quality of the probe will be indicated by the FOI (Frequency of incorporation) and the concentration of the incorporated dye.
What is the meaning FOI?FOI means “Frequency of incorporation”, indicating the incorporation rate of dye–molecules into a biomolecule (e.g., molecules dye/kb DNA). This function will be available in the methods Dye550 and Dye650, respectively.
What is the recommended FOI value of a sample (e.g., cDNA) for a sufficient signal on the microarray?This depends on the fluorescent dye. For cyanine dyes (e.g., Cy3) a FOI value of 15 but not more than 45 molecules fluorescent dyes / 1000 nucleotides is recommended.
Can I change the concentration unit for the fluorescent dye in the “DyeMethods”?No. The unit for the dye concentration is defined permanently with "pmol/μl" and can therefore not be changed in the parameters
What is the correction factor 260 and 280 in the “Dye 550/650 methods for?Many common fluorescent dyes, e.g., Cy3 and Cy5, show also little absorbance at 260 and 280 nm. This may lead to minute deviations when measuring the dye labeled DNA, RNA or protein in the BioPhotometer plus. This deviation can be corrected by a correction factor. The correction factors for Cy3, Cy5, ALEXA 555/647 are already programmed. For correction factors for other fluorescent dyes please contact the manufacturer directly.
What kind of applications can be used with the method “Assay 340”?This method is used for the evaluation of NADH based enzyme reactions. The enzymatic consumption will be analyzed via endpoint detection. NADH can be detected with the BioPhotometer plus at 340 nm. The evaluation of the results could be done via a factor, a standard or a standard curve.
d of applications can be used with the method “Assay 405”?This method is for endpoint detection of enzymatic consumption of artificial substrates. These artificial substrates serve as indicators for the activity of specific enzymes, e.g., beta-galactosidase assay or alkaline phosphatase assay. The products of the enzymatical degradation are generally para- or orthonitrophenol (pNP oder oNP). Both chemicals can be detected in the BioPhotometer plus at 405 nm. Like in the method “Assay 340” the evaluation of the results could be done via a factor, a standard or a standard curve.
What kind of applications can be used with method “Assay 490”?With the Assay 490 method cytotoxicity assays can be evaluated with the BioPhotometer plus via endpoint detection. This assay is applied for testing the vitality of living cells. The assay is based on a lactate dehydrogenase reaction combined with a specific catalyst whereas the endproduct “formazan” can be detected at 490 nm in the BioPhotometer plus. Like in the method “Assay 340” the evaluation of the results could be done via a factor,a standard or a standard curve.
Why can I change the optical pathlength to 0.2 mm in the new Biophotometer plus?With the optical pathlength of 0.2 mm microliter cell like the Hellma ®TrayCell (0.2 mm lid) can be used directly in the BioPhotometer plus without using any “virtual” dilution factors
What is the key “absorbance” in the new BioPhotometer plus for?With the absorbance key it is possible to measure the absorbance of a sample directly without using any calculation parameter.
What kind of wavelengths can be used with the BioPhotometer plus?With the BioPhotometer plus the wavelengths 230 nm, 260 nm, 280 nm, 340 nm, 405 nm, 490 nm, 550 nm, 595 nm and 650 nm are available. With the “absorbance” key, all wavelengths can be used for direct measurement without using any calculation factors. The data can be used for further processing.
How many wavelengths can be used with new BioPhotometer plus?9 wavelengths can be used with the BioPhotometer plus
Can you run kinetics with the new BioPhotometer plus in contrast to the former BioPhotometer?No, it is not possible to run kinetics in the BioPhotometer plus. However, due to the availability of the additional wavelengths many enzymatic assays can be carried out via endpoint detection.
Can I use the "Warburg-Formula" with the BioPhotometer plus for determining the protein concentration at 280 nm?No, this is unfornutately not possible, but in the result display the absorbance at 280 nm and 260 nm is shown. So it is possible to calculate the protein concetration via the "Warburg-Formula" manually: c(Protein) = 1,55 x E(280) - 0,757 x E(260). Please note that photometric measurement of the protein concentrion in the UV-range only suitable for pure and homogeneous protein solutions.
BioPhotometer™ plus: Programming of standard curves for Bradford protein determination
Simple reprogramming of the parameters on the BioPhotometer™ plus
Using the Eppendorf µCuvette™ G1.0 in the Eppendorf BioPhotometer plus® for the measurement of small sample volumes
Using the Hellma® TrayCell™ with the Eppendorf BioPhotometer plus™ - 1 mm lightpath
Using the Hellma® TrayCell™ with the Eppendorf BioPhotometer plus™ - 0.2 mm lightpath