The Eppendorf UVette® allows for measurements in the UV/VIS range with transparency from 230 nm to 1600 nm. Choose between two optical path lengths, 10 mm and 2 mm, with a single 90° rotation. The UVette is ideally matched for use with the Eppendorf BioPhotometer® plus and BioSpectrometer. For use with other spectrophotometers, the UVette can be used with the aid of an adapter.
- Suitable for measuring small volumes (≥50 µL)
- UV and VIS-transparent between 220 nm and 1,600 nm
- Volume markings for 500 µL and 1,000 µL
- Two built-in optical path lengths in a single cuvette-just turn the UVette 90° to change from 10 mm and 2 mm
- Available in individually packaged RNase-, DNA- and protein-free quality
- Deep-lying optical window prevents scratches
- Tapered cuvette base for optimal filling
- Frosted grip for labeling
- Ideal for use in the BioPhotometer plus and BioSpectrometer
- Adapters available for use with other common spectrophotometers
UV-transparent plastic, free of fluorpolymers and
other halogenated hydrocarbons
|Spectral range||220-1600 nm|
|Volume range||50-2000 µL|
|Light center height||8.5 mm|
|No. of optical
|Basic absorbance||≤0.5E at 260 nm|
|Optical pathlengths||10 and 2 mm|
|Dimensions (W x D x
|12.5 x 12.5 x 36 mm|
Application 228 BioPhotometer plus UVette Reproducible photome
Reproducible photometric determination of DNA concentrations using the Eppendorf UVette® in the Eppendorf BioPhotometer plus™
|English (US)||1.22 MB||download|
Flyer BioPhotometer plus UVette The sixty seconds sm
The sixty seconds smile effect - Eppendorf BioPhotometer ™ plus and UVette ®
|English (US)||433.12 kB||download|
What is the UVette made of?The UVette is made of a UV-transparent plastic.
Does the UVette material contain halogens (fluorine, chlorine, bromine, iodine), polystyrene, softeners, phosphorus, nitrogen or metals?No.
When do you recommend using the 2-mm light path of the UVette and when should the 10-mm light path be used?In general, the absorbance should be within the linear measuring range of the spectrophotometer (measuring range of Biophotometer: A = 0–3.0). For samples with a normal or a low concentration, the 10-mm light path is more suitable. For high-concentration samples (with A
Which reagents is the UVette resistant to, and which reagents is it not resistant to?The UVette is resistant to: - liquid solutions in the pH 0-14 range - pure acetic acid - alcohols - acetone, butanone - polar organic solvents. It is not resistant to: - hydrocarbons - chlorinated hydrocarbons - aromatics such as benzene, toluene, xylene. "Material stability UVette" http://www.eppendorf.com/uvette/en/uvette
I use your BioPhotometer and the UVette. After I measured my blank using distilled water with a cuvette optical path length of 10 mm, I measured samples (= also with distilled water) with an optical path length of both 10 mm and 2 mm . The BioPhotometer results varied, in particular for the measurements with a 2 mm optical path length. What is this due to?Please bear in mind that with a 10 mm optical path length water has a different refractive index than with an optical path length of 2 mm . As soon as you switch from 10 to 2 mm (and vice versa), you must again perform a blank measurement before sample measurement. Otherwise you will obtain incorrect measured values. Please bear in mind that every (!) cuvette is placed in the cuvette shaft in the same orientation during a series of measurements. Tip: use the orientation aids on the cuvettes, e.g. tip of arrow in top third of UVette.
I would like to use the UVette in a spectrophotometer. How can I determine which adapter I need for my device?The UVette can in principle be used in devices with which good results have already been attained using ultra micro cuvettes. To select the correct adapter, you need to know the light path height of your photometer. Under www.uvcuvette.info, we have filed a summary of "UVette adapters" (under "More information") which lists the relevant UVette adapters for numerous devices. If your device is not on the list, please ask the device manufacturer for the light path height of your device or contact our Application Hotline (Tel. +49 1803 666 789; email@example.com).
In what temperature range can the UVette be used?The material characteristics of the UVette are identical within a temperature range between room temperature and 40 °C. Measurement of samples between 80 -100 °C is not possible.
Is the UVette resistant to reagent xyz?Please take a look into our brochure „Material resistance of the Uvette“, if your reagent is listed. You will find this brochure on our homepage www.Uvcuvette.Info/ Technical Information If your reagent is not mentioned, please ask our application-hotline for assistance.(firstname.lastname@example.org)
Can I use the UVette in my spectrophotometer?Please check if your spectrophotometer is listed in our brochure „Adapter for Uvette“. You can find this brochure on our homepage www.Uvcuvette.Info/ More Information If your spectrophotometer is not mentioned, please ask our application-hotline for assistance.(email@example.com)
Can I reuse the UVette?We recommend that the UVette be discarded following the sample measurement in order to guarantee contamination-free analysis. Cleaning processes and/or multiple use of an UVette may damage the quality of the optical surfaces, particularly in the sensitive UV-range.
Can I make further use of my DNA for subsequent applications following photometric measurement?If you wish to continue to work with your DNA following the photometric determination of concentration, please observe that damages in the DNA molecule can occur during measurement as a result of exposure to UV radiation.
Can I also perform my sample measurement with an optical path length of 2 mm when I use the UVette in combination with an adapter?No. However, as with quartz glass cuvettes, it is important to make certain that the UVette is placed in the same direction in the cuvette shaft for both the blank and the sample measurement.
My application requires a sterile cuvette. Is the UVette autoclavable?No. This is because autoclaving can result in distortion of or changes to the optical characteristics of the UVette material. However, we deliver the UVette already free of DNA, RNase and protein.
Do I need to place the UVette in a certain direction in a spectrophotometer?No. However, as with quartz glass cuvettes, it is important to make certain that the UVette is placed in the same direction in the cuvette shaft for both the blank and the sample measurement.
Should the same UVette be used for the measurement of the blank and of the sample?Because the self-absorbance values of the UVettes vary somewhat from one another, ideally, the same UVette should be used for both measurements.
What does the term "light path height" mean?The light path height of a photometer informs you at what height, in relation to the bottom of a cuvette, the light beam of the photometer occurs.
What is important to know when I am working with the UVette with a minimum fill volume of 50 µl?Please make sure that there are no air bubbles in the solution and that there is no residual liquid on the inner wall of the UVette. Tip: pipette the 50 µl directly into the lower part of the UVette.
Does the UVette show an affinity toward bonding with DNA/RNA?Within the framework of photometric measurability, DNA/RNA does not collect on the surface of the UVette over a period of 15 minutes.
What are the dimensions of the individual UVette adapters?All UVette adapters have dimensions of 12.5 x 12.5 mm. These correspond to the standard dimensions for cuvettes with 1 cm optical path length.
What significance does the arrow with the embossed "10" have for the UVette?The arrow serves as an orientation aid in two ways. First, it clearly shows the 10 mm light beam of the UVette. It also makes it easier to note the selected direction of the UVette in the cuvette shaft for the blank measurement.
What is the maximum filling volume of the UVette?Up to 2,000 µl may be pipetted into the UVette.
What is the minimum filling volume of the UVette?The minimum filling volume of the UVette is 50 µl.
Are the individual UVettes blister-packed in a specific direction?Yes. Each UVette is packaged in such a way that the cuvette opening points toward the tear-strip of the blister-pack. In this way you can be sure not to touch the optical window of the UVette when tearing open the blister-pack.
What is the basic absorbance (self-absorbance) of the UVette?The self-absorbance of the UVette is
How can I avoid possible contamination of my highly-sensitive probes as a result of carrying out the previous blank measurement in the same UVette?You can do this by first measuring the sample, then rinsing the UVette thoroughly with buffer before measuring the blank. You must then subtract the blank value from your sample result.
Is a transmission curve (self-absorption of the UVette at different wavelengths) available for the UVette?Yes, this can be found in the pack insert of the UVette.http://www.eppendorf.com/uvette/en/uvette